Lysine-Appended Polydiacetylene Scaffolds for Human Mesenchymal Stem Cells
Identifieur interne : 004069 ( Main/Exploration ); précédent : 004068; suivant : 004070Lysine-Appended Polydiacetylene Scaffolds for Human Mesenchymal Stem Cells
Auteurs : V. Haridas [Inde] ; Sandhya Sadanandan [Inde] ; Pierre-Yves Collart-Dutilleul [France] ; Stan Gronthos [Australie] ; Nicolas H. Voelcker [Australie]Source :
- Biomacromolecules [ 1525-7797 ] ; 2014.
Descripteurs français
- Pascal (Inist)
- Diacétylène polymère, Polymère conjugué, Polymère fonctionnel, Fluorène dérivé polymère, Amine polymère, Préparation, Polymérisation photochimique, Polymérisation topotactique, Polymérisation phase solide, Monomère, Autoassemblage, Carbamate organique, Modification chimique, Déprotection, Morphologie, Mouillabilité, Adhérence cellulaire, Cellule souche, Cellule mésenchymateuse, Cytodifférenciation, Ostéogenèse, Effet substituant, Etude expérimentale, Polyélectrolyte, Vanadium.
- Wicri :
- topic : Cellule souche, Vanadium.
English descriptors
- KwdEn :
- Amine polymer, Cell adhesion, Cell differentiation, Chemical modification, Conjugated polymer, Deprotection, Diacetylene polymer, Experimental study, Fluorene derivative polymer, Functional polymer, Mesenchymal cell, Monomer, Morphology, Organic carbamate, Osteogenesis, Photopolymerization, Polyelectrolyte, Preparation, Self assembly, Solid polymerization, Stem cell, Substituent effect, Topochemical polymerization, Wettability, vanadium.
Abstract
We report on the self-assembly based fabrication of fibrous polymers for tissue engineering applications. Directed self-assembly followed by polymerization of lysine-appended diacetylenes generated a variety of polymers (P1- PS) with distinct chemical properties. The self-assembly along with the conjugated double and triple bonds and rigid geometry of diacetylene backbone imposed a nanofibrous morphology on the resulting polymers. Chemical properties including wettability of the polymers were tuned by using lysine (Lys) with orthogonal protecting groups (Boc and Fmoc). These Lys-appended polydiacetylene scaffolds were compared in terms of their efficiency toward human mesenchymal stem cells adhesion and spreading. Interestingly, polymer P4 containing Lys Nα-NH2 and Lys Nε-Boc with balanced wettability supported cell adhesion better than the more hydrophobic polymer P2 with Nε-Boc and Nα-Fmoc or more hydrophilic polymer P5 containing free Nε and Nα amino groups. The molecular level control in the fabrication of nanofibrous polymers compared with other existing methods for the generation of fibrous polymers is the hallmark of this work.
Affiliations:
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Haridas</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, Indian Institute of Technology Delhi</s1><s2>New Delhi-110016</s2><s3>IND</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Inde</country><wicri:noRegion>New Delhi-110016</wicri:noRegion></affiliation></author><author><name sortKey="Sadanandan, Sandhya" sort="Sadanandan, Sandhya" uniqKey="Sadanandan S" first="Sandhya" last="Sadanandan">Sandhya Sadanandan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, Indian Institute of Technology Delhi</s1><s2>New Delhi-110016</s2><s3>IND</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Inde</country><wicri:noRegion>New Delhi-110016</wicri:noRegion></affiliation></author><author><name sortKey="Collart Dutilleul, Pierre Yves" sort="Collart Dutilleul, Pierre Yves" uniqKey="Collart Dutilleul P" first="Pierre-Yves" last="Collart-Dutilleul">Pierre-Yves Collart-Dutilleul</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Bio-Nano Laboratory, Faculty of Dentistry, Montpellier University</s1><s2>Montpellier</s2><s3>FRA</s3><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Gronthos, Stan" sort="Gronthos, Stan" uniqKey="Gronthos S" first="Stan" last="Gronthos">Stan Gronthos</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Centre for Stem Cell Research, Robinson Institute</s1><s2>North Adelaide , SA-5006</s2><s3>AUS</s3><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>North Adelaide , SA-5006</wicri:noRegion></affiliation></author><author><name sortKey="Voelcker, Nicolas H" sort="Voelcker, Nicolas H" uniqKey="Voelcker N" first="Nicolas H." last="Voelcker">Nicolas H. Voelcker</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Mawson Institute, University of South Australia</s1><s2>Adelaide, SA-5001</s2><s3>AUS</s3><sZ>5 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Adelaide, SA-5001</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Biomacromolecules</title><title level="j" type="abbreviated">Biomacromolecules</title><idno type="ISSN">1525-7797</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Biomacromolecules</title><title level="j" type="abbreviated">Biomacromolecules</title><idno type="ISSN">1525-7797</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amine polymer</term><term>Cell adhesion</term><term>Cell differentiation</term><term>Chemical modification</term><term>Conjugated polymer</term><term>Deprotection</term><term>Diacetylene polymer</term><term>Experimental study</term><term>Fluorene derivative polymer</term><term>Functional polymer</term><term>Mesenchymal cell</term><term>Monomer</term><term>Morphology</term><term>Organic carbamate</term><term>Osteogenesis</term><term>Photopolymerization</term><term>Polyelectrolyte</term><term>Preparation</term><term>Self assembly</term><term>Solid polymerization</term><term>Stem cell</term><term>Substituent effect</term><term>Topochemical polymerization</term><term>Wettability</term><term>vanadium</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Diacétylène polymère</term><term>Polymère conjugué</term><term>Polymère fonctionnel</term><term>Fluorène dérivé polymère</term><term>Amine polymère</term><term>Préparation</term><term>Polymérisation photochimique</term><term>Polymérisation topotactique</term><term>Polymérisation phase solide</term><term>Monomère</term><term>Autoassemblage</term><term>Carbamate organique</term><term>Modification chimique</term><term>Déprotection</term><term>Morphologie</term><term>Mouillabilité</term><term>Adhérence cellulaire</term><term>Cellule souche</term><term>Cellule mésenchymateuse</term><term>Cytodifférenciation</term><term>Ostéogenèse</term><term>Effet substituant</term><term>Etude expérimentale</term><term>Polyélectrolyte</term><term>Vanadium</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Cellule souche</term><term>Vanadium</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report on the self-assembly based fabrication of fibrous polymers for tissue engineering applications. Directed self-assembly followed by polymerization of lysine-appended diacetylenes generated a variety of polymers (P1- PS) with distinct chemical properties. The self-assembly along with the conjugated double and triple bonds and rigid geometry of diacetylene backbone imposed a nanofibrous morphology on the resulting polymers. Chemical properties including wettability of the polymers were tuned by using lysine (Lys) with orthogonal protecting groups (Boc and Fmoc). These Lys-appended polydiacetylene scaffolds were compared in terms of their efficiency toward human mesenchymal stem cells adhesion and spreading. Interestingly, polymer P4 containing Lys N<sup>α</sup>-NH<sub>2</sub> and Lys N<sup>ε</sup>-Boc with balanced wettability supported cell adhesion better than the more hydrophobic polymer P2 with N<sup>ε</sup>-Boc and N<sup>α</sup>-Fmoc or more hydrophilic polymer P5 containing free N<sup>ε</sup> and N<sup>α</sup> amino groups. The molecular level control in the fabrication of nanofibrous polymers compared with other existing methods for the generation of fibrous polymers is the hallmark of this work.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>Inde</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li></settlement></list><tree><country name="Inde"><noRegion><name sortKey="Haridas, V" sort="Haridas, V" uniqKey="Haridas V" first="V." last="Haridas">V. Haridas</name></noRegion><name sortKey="Sadanandan, Sandhya" sort="Sadanandan, Sandhya" uniqKey="Sadanandan S" first="Sandhya" last="Sadanandan">Sandhya Sadanandan</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Collart Dutilleul, Pierre Yves" sort="Collart Dutilleul, Pierre Yves" uniqKey="Collart Dutilleul P" first="Pierre-Yves" last="Collart-Dutilleul">Pierre-Yves Collart-Dutilleul</name></region></country><country name="Australie"><noRegion><name sortKey="Gronthos, Stan" sort="Gronthos, Stan" uniqKey="Gronthos S" first="Stan" last="Gronthos">Stan Gronthos</name></noRegion><name sortKey="Voelcker, Nicolas H" sort="Voelcker, Nicolas H" uniqKey="Voelcker N" first="Nicolas H." last="Voelcker">Nicolas H. Voelcker</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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